Table 1.

Media composition (mM/ml)

	T6	HTF
NaCl	80.77	101.61
KCL	1.48	4.69
NaH2PO4	0.39	–
KH2PO4	–	0.40
MgCl2	0.49	–
MgSO4	–	0.20
CaCl2	1.77	5.14
NaHCO3	25.00	25.00
Na lactate	23.29	18.36
Na pyruvate	0.27	0.34
d‐glucose	5.55	2.70
Penicillin a	62.80	62.80
Streptomycin a	50.00	50.00
Phenol red	0.01	0.01
HEPES	–	2.49
BSA a	15.00	4.00
EDTA	0.02	0.01

^aMaterials are in mg/ml