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. 2018 Apr 4;2018:4851949. doi: 10.1155/2018/4851949

Figure 2.

Figure 2

The effect of exosomal miRNA on colony formation in vitro. To evaluate the function of miR-328-3p and miR-503-3p on cancer stem-like properties, a colony formation assay was performed using four different cancer cell lines including MCF7, BT-474, HCT-15, and COLO 205. (a) The numbers of colonies are shown using cells transfected with 20 nM miR-328-3p and miR-503-3p. Treatment with miRNA-NC was used as negative control. Inserts indicate magnified images. (b) Colony numbers were quantified using ImageJ. The number of colonies, formed by cells transfected with miR-503-3p, was significantly lower than that formed by cells treated with negative control. ∗∗ P < 0.01 by a one-way ANOVA followed by Tukey's test.