Figure 5.

The effect of Tiron on profilin-1-mediated RhoA/ROCK1 activation in HUVECs. HUVECs stably overexpressing profilin-1 were treated with 10 μM Tiron 12 h before application of 50 μg/ml AGEs. (a) Western blot analysis of profilin-1, T-RhoA, p-RhoA, p-eNOS, and ROCK1 in profilin-1 overexpression HUVECs treated with Tiron under 50 μg/ml AGEs. (b) Intracellular ROS, (c) apoptosis, (d) tube formation, and (e) migratory abilities were analyzed in profilin-1 overexpression HUVECs treated with Tiron under 50 μg/ml AGEs. ^∗∗P < 0.01