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. 2018 Apr 17;15:109. doi: 10.1186/s12974-018-1137-1

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — NLRP3 priming was induced by LPS stimulation. Different doses of LPS were added to the cell culture media of BV-2 cells or primary microglial cultures for 30 min. a The mRNA of NLRP3 was quantified by real-time qPCR immediately after LPS stimulation. Values are expressed as fold changes over the mean values of blank control. b IL-1β concentration in the supernatant was measured 6 h later. All results are presented as mean ± SD (n ≥ 3). *P < 0.05 and **P < 0.01 compared with the corresponding data of blank control