Fig. 4.

Isoflurane-induced NLRP3 inflammasome activation after priming. BV-2 microglia were exposed to 4% isoflurane for 6 h in the cells primed with or without 1 μg/mL LPS. Primary microglial cultures were exposed to 2% isoflurane for 6 h in the cells primed with or without 5 ng/mL LPS. Control = blank control; ISO = Isoflurane exposure; NLRP3-primed = LPS stimulation; NLRP3-primed+ ISO = NLRP3-primed + isoflurane exposure. a Western blot images of NLRP3, caspase-1 P45, caspase-1 P20, ASC, IL-18, and IL-1β from BV-2 cells. b–g Graphic presentation of abundance of each protein in BV-2 cells. h Western blot images of NLRP3, ASC, caspase-1 P45, and caspase-1 P20, IL-18, and IL-1β. i–n Graphic presentation of abundance of each protein in primary microglia. Values are expressed as fold changes over the mean values of control and are presented as mean ± SD (n ≥ 3). *P < 0.05 and **P < 0.01 compared with the corresponding data of group control. #P < 0.05 and ##P < 0.01 compared with the corresponding data of group NLRP3-primed