Table 1.
Target | Forward primer (5'-3') | Reverse primer (5'-3') | Product size (bp) |
---|---|---|---|
Caspase 3 | GACAGACAGTGGTGTTGATG | TGGATGAACCAGGAGCCATC | 132 |
Caspase 8 | AACCTGGTACATCCAGTCAC | AAAGTAGGCTGAGGCATCTG | 150 |
Caspase 9 | AAGGTTTGAGGACCTTCGAC | GACTGCAGGTCTTCAGAGTG | 184 |
Caspase 4 | GTGGAGAAGGACTTCATTGC | CTGGAAGCATGTGATGAGTTG | 108 |
ATF4 | CAGCTACCACCCATAACAAG | GTGTCCATCACCTGACAGTC | 138 |
ATF6 | CAGCTACCACCCATAACAAG | GTGTCCATCACCTGACAGTC | 133 |
GRP78 | GTCCTTCTATGAAGGAGAAG | GAATCTTCCAACACTTTCTGG | 117 |
CHOP | TGCAAGAGGTCCTGTCTTCAG | GCACTGACTCCTCGGAAC | 106 |
XBP1 | AAGGCGCTGAGGAGGAAAC | GGTTCTCAACTACAAGGCC | 178 |
GAPDH | CTTCATTGACCTCAACTACATGG | CTCGCTCCTGGAAGATGGTGAT | 134 |
Abbreviations: ATF activating transcription factor, GRP78 78-kDa glucose-regulated protein, CHOP C/EBP homologous protein, XBP1 X-box binding protein-1, GAPDH glyceraldehyde-3-phosphate dehydrogenase