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. 2018 Apr 17;37:84. doi: 10.1186/s13046-018-0746-y

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© The Author(s). 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Analysis of the biochemical effects exerted by RET-G533C and RET-P1047S mutants. Immunoblot analysis of RET expression and activity in transfected HEK293 cells (a). Lysates from transfected HEK293 cells were analysed by immunoblot with anti-phosphoY1062 or anti-RET antibody. The activation status of the MAPK pathway was assessed with anti phospho-Y202T204 ERK1/2 antibody. Anti-ERK1/2 antibody was used for normalization. b. Immunoblot analysis of RET dimers in transfected HEK293 cells. Samples were prepared under non-reducing (NR) or reducing (R) conditions to detect RET dimer formation and loaded onto SDS-PAGE in non-reducing conditions. Half the total amount of protein lysate was loaded in the case of RET634