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. 2018 Apr 18;14:31. doi: 10.1186/s13007-018-0299-2

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Dual visualization of choline phospholipids and GFP marker lines. Arabidopsis seeds were germinated and seedlings were harvested for click chemistry after 7 days on agar media containing 200 µM propargylcholine. a–d Alexa Fluor 594 azide (red) indicates propargyl-PC, a′–d′ GFP (or YFP) (green) marks a specified intracellular compartment, and a″–d″ merged images indicate overlapping expression as yellow or orange, as imaged by confocal laser scanning microscopy. a Hypocotyl epidermal cell shows localization of a mitochondrial marker in comparison to propargyl-PC; b–d root epidermal cells indicate localization of markers for b endoplasmic reticulum (ER), c Golgi apparatus, and d tonoplast. Co-localization was quantified using the Pearson correlation coefficient (PCC) (see Additional file 3: Table S1)