Fig. 7.

Quantification of propargyl-PC versus PC in plant tissues. Choline lipids were determined by ESI-MS/MS (see “Methods”; Additional files 4 and 5: Tables S2 and S3). PC (grey) and propargyl-PC (red) are represented in stacked graphs showing the replacement of PC by propargyl-PC in treated a whole seedlings, and b–f tissues from mature/reproductive plants: b roots, c leaves, d stems, e cotyledons, and f siliques. Treated seedlings and mature plants were grown in media supplemented with 250 and 200 µM propargylcholine, respectively. Controls were grown in media that lacked propargylcholine. Error bars indicate standard deviations for n = 5. No significant differences were found between the total PC + propargyl-PC versus PC in the treated and control samples, respectively (Two-tailed t-test, P < 0.005)