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. 2018 Apr 11;5:57. doi: 10.3389/fvets.2018.00057

Figure 3.

Figure 3

Tylvalosin has no significant effect on resting cell necrosis in vitro. Secreted lactate dehydrogenase (LDH) was measured to assess cell necrosis, by quantifying the amount of LDH protein in cell culture supernatants. Porcine neutrophils were incubated with control (10% FBS in HBSS), 10 μg/mL tylvalosin, equimolar concentrations of lincomysin (linco; 11 μM) and 1% Triton X-100 (positive control). These values were then expressed as a fold change compared to the vehicle at (A) 0.5 and (B) 1 h. Values are mean ± SEM (n = 9), **P < 0.01. (C) Cell necrosis (measured by LDH release) in porcine monocyte-derived macrophages incubated with control (10% FBS in IMDM), tylvalosin (0.1, 1, or 10 μg/mL), or 1% Triton X-100 (positive control) for 0.5–24 h. Values were expressed as a fold change compared as a ratio to control. Values among controls and tylvalosin-treated cells did not differ among each other (n = 6–9/group). Data are mean ± SEM, *P < 0.05 versus control.