Figure 6.

Tylvalosin treatment inhibits CXCL8 and IL-1α protein secretion in LPS-stimulated macrophages. Porcine macrophages were treated with control vehicle (10% HI-FBS in IMDM) or tylvalosin (10 μg/mL), in the presence of absence of LPS (1 μg/mL) for 2 h. Supernatants were collected and submitted to multiplex analysis of secreted chemokines/cytokines. Using this bead-based fluorescent assay and the standard curves it generates secreted protein was measured and values are expressed as picograms/milliliter. CXCL8 (A) and IL-1α (B) protein secretion was inhibited by tylvalosin in LPS-stimulated macrophages. Data represent mean ± SEM (n = 3–4/group). *P < 0.05, **P < 0.01 versus unstimulated control; ^#P < 0.05 versus LPS-stimulated macrophages.