Figure 7.

Tylvalosin promotes the secretion of the pro-resolution lipid mediators LXA₄ and RvD₁. Secreted levels of LXA₄ (A), its metabolite precursor 15(S)-HETE (B), and RvD₁ (C) were measured from porcine neutrophils treated with 10% HI-FBS in HBSS (control) or with 10 μg/mL tylvalosin for 0.5 h using liquid chromatography–mass spectrometry (LC/MS). Values represent mediator levels in pM calculated from standard curves generated by integrating the area under the curve of elution peaks where intensity correlated with known LXA₄, 15(S)-HETE, or RvD₁ concentrations, respectively. Data represent mean ± SEM (n = 18–20). *P < 0.05, ***P < 0.01 versus unstimulated control.