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Morphological characterization of a standard AFM calibration grating with and without flattening. (a) The raw AFM image of the standard calibration grating. (b) Flattened image of the grating. (c) Comparison of cross sections of a micrometer-sized pit before and after the flattening. The blue curve shows the profile in raw image, while the red curve shows the profile in flattened image. The flattened image gives a better measurement of the depth of the pit. The scale bar is 10 µm.
