Table 2.

In vitro cytostatic effects of GnRH-III bioconjugates on HT-29 human colon cancer and MCF-7 human breast cancer cell line.

code	[8Lys(Dau=Aoa)]–GnRH-III compound	MCF-7 IC50 [µM]	HT-29 IC50 [µM]
K1	[6L-Asp]	3.2 ± 0.1	1.5 ± 0.5
1	[6D-Asp]	13.0 ± 0.5	8.9 ± 1.3
2	[6D-Glu]	6.8 ± 1.0	10.1 ± 1.4
3	[6D-Trp]	n.d.a	n.d.a
K2	[4Lys(Bu),6L-Asp]	2.7 ± 0.1	1.9 ± 0.7
4	[4Lys(Bu),6D-Asp]	6.2 ± 0.2	9.3 ± 1.1
5	[4Lys(Bu),6D-Glu]	7.0 ± 1.2	13.7 ± 2.6
6	[4Lys(Bu),6D-Trp]	n.d.a	n.d.a

^an.d. - no data (compound 3 and 6 precipitated in medium at concentrations higher than 20 µM – no dose response). All values represent mean ± SE.