Movie S1.
Transient nuclear entry and recovery of GFP::α tubulin in lamin-N209K mutant embryos selectively depleted of endogenous lamin, related to Figure 2 and Figure S2. Confocal fluorescence images of embryos expressing GFP::α-tubulin (green) and mCherry::Histone2B (red). Embryos expressing the lamin-WT transgene (left)and lamin-N209K transgene (right) are shown. Sequences start during sperm-derived pronuclear positioning at the posterior end of the embryo and end at completion of pronuclear rotation. WT-lamin embryos exclude soluble GFP::α-tubulin from the nuclear interior until pronuclear rotation is complete. (Left panel, arrow marks entry ofGFP::α-tubulin into the nucleus). GFP::α-tubulin transiently enters the sperm-derived pronucleus in lamin-N209K mutant embryos prior to pronuclear migration(Right panel, arrows mark GFP::α-tubulin entry into pronucleus). The oocyte-derived pronucleus goes in and out of the focal plane in this embryo. The oocyte-derived pronucleusand sperm-derived pronucleus deform and lose their integrity upon pronuclear meeting and upon pronuclear rotation respectively at which time GFP::α-tubulin enters the pronucleus. Images were acquired every 20s. Playback rate is 80X real time.