Skip to main content
. 2000 Jul;123(3):905–916. doi: 10.1104/pp.123.3.905

Figure 7.

Figure 7

Immunodetection of CHRK1 protein. A, Immunoblotting of the recombinant CD with anti-CHRK1 antiserum. Ten micrograms of protein extracts from uninduced (UI) and induced (I) E. coli cells that carry the pET32a-CD plasmid was subjected to immunoblotting. The recombinant CHRK1 is marked with the arrowhead. B, Immunodetection of CHRK1 proteins in membranes of tobacco BY2 cells. Thirty micrograms of soluble (S) and membrane (M) proteins from BY2 cells was subjected to immunoblotting. The CHRK1 protein is marked with the arrowhead. Molecular mass markers are indicated in kD. C, Immunodetection of CHRK1 protein expressed in animal cells. CHRK1 protein was transiently expressed in human 293T cells under the control of CMV promoter. Thirty micrograms of S and M proteins isolated from transfected cells with pCMV or pCMV-CHRK1 was subjected to immunoblotting. The expressed CHRK1 protein is marked with the arrowhead. Molecular mass markers are indicated in kD.