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Use of EN method to evaluate the effects of different experimental strategies and parameters on precision (i.e., within-group variability, CV %) and quantitative accuracy(i.e., deviation from true ratio, 0 at log 2 scale). (A,B) Effects of different quantitative strategies including ion current (IC)-based methods (median-IC, LMM-IC, and Sum-IC) and MS2-based methods (MS2-TIC and SpC). (C,D) Effects of the quality of chromatographic separation and (E,F) effects of database selection.
