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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Biochem Pharmacol. 2017 Nov 15;147:67–76. doi: 10.1016/j.bcp.2017.11.005

Fig. 4.

Fig. 4

The Nrf2 activators, CDDO-Im and tBHQ have differential effects on TNFα secretion. Freshly isolated splenocytes from Nrf2-null and wild-type mice were either left untreated (BKG) or were pretreated with vehicle (VEH, 0.01% ethanol for tBHQ or 0.01% DMSO for CDDO-Im), tBHQ, or CDDO-Im for 30 min prior to activation with anti-CD3/anti-CD28. After 24 h, the cell supernatants from A.) tBHQ-treated splenocytes or B.) CDDO-Im treated splenocytes were collected and TNFα was quantified by sandwich ELISA. a = p<0.05 versus wild-type VEH, b = p<0.05 versus Nrf2-null VEH, c = p<0.05 between the wild-type and Nrf2-null genotypes.