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. 2018 Mar 19;159(5):2062–2074. doi: 10.1210/en.2018-00117

Figure 4.

Figure 4.

The difference in RA synthesis and degradation enzymes between cumulus cells and granulosa cells. (A) The expression of Adh1, Adh5, Aldh1a1, Aldh1a7, and Cyp26b1 mRNA in granulosa cells of immature mice treated with or without eCG in vivo. The value of eCG at 0 hours in granulosa cells was set as 1, and the data are presented as fold induction. Values are given as mean ± standard error of the mean (SEM) of three replicates. Significant differences were observed in comparison with the value of eCG at 0 hours (P < 0.05). (B) The expression of Adh1, Adh5, Aldh1a1, Aldh1a7, and Cyp26b1 mRNA in cumulus cells of immature mice treated with or without eCG in vivo. The value of eCG at 0 hours in cumulus cells was set as 1, and the data are presented as fold induction. Values are given as mean ± SEM of three replicates. Significant differences were observed in comparison with the value of eCG at 0 hours (P < 0.05). (C) Activity of LacZ enzyme driven by RARE promoter in cumulus cells and granulosa cells of immature mice with or without 48-hour eCG treatment. Values are shown for RARE-LacZ activity at 595 nm after the protein samples were incubated for 2 hours at 37°C with chlorophenol red-β-d-galactopyranoside. Values are given as mean ± SEM of more than three replicates. eCG treatment significantly increased RARE-LacZ activity in granulosa cells. *P < 0.05.