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. 2018 Apr 11;9:339. doi: 10.3389/fphys.2018.00339

Figure 4.

Figure 4

Ammonia transporter expression in the anal papillae of larval A. aegypti. (A) RT-PCR amplicon of AeAmt2, the corresponding no template control (NTC) for AeAmt2 primers, AeRh50-2, AeRh50-1, and AeAmt1 expression in the anal papillae resolved by gel electrophoresis. (B) Comparison of AeAmt2, AeRh50-1, AeRh50-2, and AeAmt1 mRNA abundance in the anal papillae. Each gene was normalized to 18s ribosomal RNA abundance in the anal papillae and was expressed relative to AeRh50-1 (assigned a value of 1). The dashed line illustrates y = 1. Data are expressed as mean values ± SEM (n = 3). Letters denote significant differences in relative mRNA abundance based on a One-way ANOVA (Holm-Sidak for multiple comparisons) of log transformed values (p = 0.00002). (C) Representative Western blot of larval anal papillae homogenates probed with AeAmt2 antisera revealing a single putative monomer at ~55 kDa (top). The single 55 kDa band was blocked by antibody pre-absorption with the immunogenic peptide. Coomassie total protein staining, used as a loading control, is shown in the lower.