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. 2018 Apr 11;9:339. doi: 10.3389/fphys.2018.00339

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Copyright © 2018 Durant and Donini.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of high environmental ammonia (HEA) on transcript abundance of ammonia transporter genes in the anal papillae of A. aegypti larvae. Relative mRNA abundance of (A) AeAmt1, (B) AeAmt2, (C) AeRh50-1, and (D) AeRh50-2 at 6 and 48 h post transfer to dechlorinated tap water (deH₂O) or HEA (5mM NH₄Cl) (n = 3). Each gene was normalized to 18s ribosomal RNA abundance and expressed relative to levels in the control deH₂O group (assigned a value of 1). Data is shown as mean values ± SEM. Asterisks denote statistically significant differences between deH₂O and HEA groups (Unpaired t-test, p ≤ 0.05).