Figure 9.

Effects of high environmental ammonia (HEA) exposure on protein abundance of ammonia transporter genes in the anal papillae of fourth instar A. aegypti larvae. (A) AeAmt1 normalized monomer (30 kDa) abundance, (B) AeAmt2 normalized monomer (55 kDa) abundance, and (C) AeRh50s normalized monomer (48 kDa) abundance, and corresponding representative Western blots (right panels, 1 representative of three replicates) at 6, 48 h, and 7 days in dechlorinated tap water (deH₂O) or HEA (5 mM NH₄Cl) (n = 3). For 6 and 48 h HEA exposure, protein abundance was normalized to GAPDH abundance in the anal papillae. For 7-day HEA exposure, protein abundance was normalized to total protein (Coomassie). Normalized protein abundance for each protein is expressed relative to the control deH₂O group (assigned a value of 1). Data is shown as mean values ± SEM. Asterisks denote statistically significant differences between deH₂O and HEA groups ($$unpaired t-test, ^*p ≤ 0.05, ^**p ≤ 0.005).