Figure 7.

Gelatin zymography analysis of Cs1-MMP activity. An aliquot (10 L) of the undiluted refolded enzyme (lanes 1 and 4) and of a range of dilution (1:5, lanes 2 and 5; 1:50, lanes 3 and 6) was fractionated by electrophoresis in a 15% (w/v) SDS-acrylamide gel containing type-I collagen as a gelatin substrate. An aliquot of the overexpressed but not refolded protein was also loaded as a control (C). The gel was cut in half and incubated overnight in the assay buffer containing either dimethyl sulfoxide as a control (A) or 3 m BB94 (B).