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. 2018 Apr 5;23(1):90–99. doi: 10.1016/j.celrep.2018.03.027

Figure 4.

Figure 4

EM Images of HA-Antibody Complexes

(A and B) Reference -free class averages of (A) unliganded HA and (B) HA bound to antibody 3.1 IgG1.

(C–E) Representative class averages of HA bound to IgG1 (C), filtered image with enhanced contrast (D), and filtered image with docked crystal structures (PDB: 4PY8) in blue and green (E). Two HAs are cross-linked by the two arms of the antibody and are oriented in anti-parallel fashion.

(F–H) Representative class averages of HA bound to 3.1 IgA1 (F), filtered image with enhanced contrast (G), and filtered image with docked crystal structures (PDB: 4PY8 and 3CHN) in blue and green (right) (H). Two HAs are oriented so that their head domains are close to one another in a head-to-head fashion. The additional density between the heads likely corresponds to the Fc domain (yellow). Arrows indicate the orientation of the HA, with the head region at the top of the arrow. Scale bar in (F), 10 nm.