Effects of E2 and TX on Mn-induced decrease of GLAST and GLT-1 in the cerebellar region of the mouse brain. At the end of treatment with Mn, E2, and TX, cerebellar tissues were prepared from the mouse brain as described in the Methods section. Tissues were analyzed for mRNA and protein levels of GLAST (A and B) and GLT-1 (C and D). GAPDH and β-actin were used as loading controls of mRNA and protein, respectively. #, p<0.05; ###, p<0.001; *, p<0.05; **, p<0.01; ***, p<0.001; @, p<0.05;, p<0.01; @, p<0.001 compared to control (two-way ANOVA followed by Tukey’s post hoc test; n = 3).