Effects of E2 and TX on Mn-induced oxidative stress in the mouse brain. At the end of treatment with Mn, E2, and TX, tissue samples were prepared from the mouse brain as described in the Methods section. Tissues were analyzed for CAT protein levels (A) and CAT activity (B), GSH levels (C) and MDA levels (D). ##, p<0.01; ###, p<0.001; *
p<0.05; **, p<0.01; ***, p<0.001 (two-way ANOVA followed by Tukey’s post hoc test; n = 3).