Effects of E2 and TX on Mn-induced apoptosis in the mouse brain. At the end of treatment with Mn, E2, and TX, tissue samples were prepared from the mouse brain as described in the Methods section. Tissues were analyzed for pro-apoptotic Bax and antiapoptotic Bcl-2 protein (A), and the levels of Bax/Bcl-2 ratio (B). β-actin was used as a loading control. ###, p<0.001; *, p<0.05 (two-way ANOVA followed by Tukey’s post hoc test; n = 3).