TABLE 2.
Fetal Resorptions and Axial Skeletal Analysis of Gestational Day 18 Mouse Fetuses Exposed to ADBAC+DDAC Disinfectant for 8 Weeks and Throughout Breeding and Gestation
| Measurement | ADBAC+DDAC concentration | |||||
|---|---|---|---|---|---|---|
| 0 | 60 | 120 | ||||
| Total resorptions | 9.4 | (2.7) | 8.5 | (2.0) | 17.1 | (2.7) |
| Early resorptions | 6.4 | (2.3) | 2.3 | (1.1) | 4.7 | (2.9) |
| Late resorptions | 3.0 | (2.2) | 6.2 | (2.1) | 12.4* | (2.3) |
| # Cervical vertebrae | 7 | (0) | 7 | (0) | 7 | (0) |
| # Thoracic vertebrae | 13 | (0) | 13 | (0) | 13 | (0) |
| # Lumbar vertebrae | 6 | (0) | 6 | (0) | 6 | (0) |
| # Sacral vertebrae | 4 | (0) | 4 | (0) | 4 | (0) |
| # Sternal ossification centers | 6 | (0) | 5.9 | (0.1) | 6 | (0) |
| % Tuberculi anterior on C7 - R | 0 | (0) | 0 | (0) | 0 | (0) |
| % Tuberculi anterior on C7 - L | 0 | (0) | 0 | (0) | 8.3 | (8.3) |
| Total ribs counted | 26.2 | (0.1) | 26.3 | (0.2) | 26.7 | (0.2) |
| % Cervical ribs - R | 5.0 | (5) | 8.3 | (8.3) | 8.3 | (8.3) |
| % Cervical ribs - L | 10.0 | (6.7) | 0.0 | (0) | 16.7 | (10.5) |
| % Total cervical ribs | 15.0 | (10.7) | 8.3 | (8.3) | 25.0 | (11.2) |
| % Lumbar rib ossification center–R | 0.0 | (0) | 8.3 | (8.3) | 25.0* | (11.2) |
| % Lumbar rib ossification center– L | 5.0 | (5) | 16.7 | (10.5) | 16.7 | (10.5) |
Resorptions were characterized as early or late based on size; mummified fetuses were included as late resorptions. Fetuses were cleared in potassium hydroxide and stained with alcian blue and alizarin red. Values represent the mean (SEM). For resorptions, N = 10–11; for skeletal analysis, N = 10, 6, and 6 for 0, 60, and 120 mg/kg/day, respectively. Late resorptions and lumbar rib ossification centers on the right (R) at 120 mg/kg/day were significantly different than controls (ANOVA p ≤ 0.05).