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. 2018 Feb 14;27(9):1533–1544. doi: 10.1093/hmg/ddy060

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Published by Oxford University Press 2018. This work is written by US Government employees and is in the public domain in the US.

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Figure 2. — Characterization of conditional deletion and rescue alleles. (A and B) Extracts prepared from adult whole hearts were analyzed by western blot for Casq2 and Junctin to detect the effects of tamoxifen induction in Casq2Flox/Casq2Flox Myh6-CreMER (conditional deletion) and Casq2RevFlox/Casq2RevFlox Myh6-CreMER (conditional rescue) models. GAPDH was analyzed as a loading control. (C) IHC staining tested CCS specificity in the Casq2RevFlox/Casq2RevFlox Hcn4KiT-Cre (conditional CCS rescue) model. Adjacent sections were either stained for Casq2 or for CCS marker Contactin2 (Cntn2). Note that Casq2 protein is detected only in Cntn2 staining regions such as the SA node. The right panels are insets shown at higher magnification to demonstrate Casq2 rescue in the AV node and Purkinje fibers. See Supplementary Material, Figure S3 for additional analyses and demonstration of antibody specificity. RV, right ventricle; LV, left ventricle.