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. 2018 Feb 24;27(9):1618–1629. doi: 10.1093/hmg/ddy069

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© The Author(s) 2018. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Analysis of redox state in Pink1 and parkin deficient flies. (A) Pseudocolour representative images of adult Drosophila brains imaging cytosolic (H₂DCFDA) or mitochondrial (mt-roGFP2-Orp1) ROS reporters in control (w¹¹¹⁸), park²⁵ or Pink1^B9 adult brains. Scale bars = 100 µm. (B) Quantification of cytosolic (H₂DCFDA) ROS normalised to control; control n = 10, park²⁵ n = 9 and Pink1^B9 n = 9. (C) Quantification of mitochondrial (mt-roGFP2-Orp1) ROS normalised to control; control n = 16, park²⁵ n = 20 and Pink1^B9 n = 16. Charts show mean ± SD. Statistical analysis used Kruskal–Wallis with Dunn’s multiple comparisons test (*P < 0.05; ns, non-significant).