Skip to main content
. 2018 Jan 18;4(4):FSO282. doi: 10.4155/fsoa-2017-0115

Figure 2. . Thermal cycling protocol for monochrome multiplex quantitative polymerase chain reaction on Real-Time PCR machine (IQ5, Bio-Rad).

Figure 2. 

In the first step, Taq DNA polymerase was activated and genomic DNA was denatured. In second step, primers were annealed and extended effectively at low temperature in two cycles. In third step, the cycles were repeated. The acquisition at 74°C provided the Ct values for telomeres and the acquisition at 88°C provided the Ct values for the single-copy gene (albumin). In the final step, melt curve was prepared by increasing temperature at the end of the experiment.

Tem.: Temperature.