Figure 1.

cPLA₂α gene disruption in subfornical organ (SFO) of cPLA₂α^+/+ mice with adenovirus (Ad)-green fluorescence protein (GFP)-cPLA₂α shirt hairpin (sh) RNA (Ad-cPLA₂α shRNA) abrogates Ang II-induced increase in blood pressure (BP) and cPLA₂ phosphoimmunoreactivity. Ad-GFP scramble (Scr) shRNA (Ad-Scr shRNA) or Ad-cPLA₂α shRNA was transduced into SFO. (a) systolic blood pressure (SBP) was measured by tail-cuff in BALB/c mice. (b) Mean arterial blood pressure (MAP) was measured by radio telemetry in C57BL/6 mice. (c) Expression of cPLA₂, and its activity measured by its phosphorylation in SFO of BALB/c mice by immunohistochemical method. Scale bars: 50 µm. (d) Quantified data. Data are expressed as mean ± SEM. n = 5 per group. *, **P <0.05, Ad-Scr shRNA-Ang II vs. Ad-Scr shRNA-Veh (Vehicle); ^†P < 0.05, Ad-cPLA₂α shRNA-Ang II vs. Ad-Scr shRNA-Ang II in cPLA₂α^+/+ BALB/c mice (a) and cPLA₂α^+/+ C57BL/6 mice (b). ^#P < 0.05, Ad-cPLA₂α shRNA vs. Ad-Scr shRNA.