Extended Data Figure 3. Developmental potential of ESCs cultured in S/L and 2i/L.

(a) Representative image of a chimeric pup derived from female ESCs cultured in S/L (left) compared to a pup obtained through natural mating (right). Body weight of each pup was shown below.

(b) Representative image of a low-grade adult chimera with patches of agouti hairs generated using female ESCs in S/L (white arrow). Her agouti germline offspring (red arrowhead) was generated by crossing her to a C57B6 wild-type mouse.

(c) Representative image of all-ESC adult mice generated from male ESCs cultured in S/L for 10 passages.

(d) Bar graphs showing percentages of transferred 4n blastocysts that survived to birth (“Full-term”), established regular breathing (“Breathing”) or survived past 5 weeks (“Adult”) using male ESCs grown in either S/L (blue bars) or 2i/L (red bars) until passage 20. The number of animals obtained per total number of transferred embryos is shown.

(e) Representative images of all-ESC neonates produced from male ESCs cultured in S/L (left) and 2i/L (middle) at passage 20. Representative image of all-ESC adult mice generated from ESCs cultured in S/L at passage 20 (right).