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In situ localization of Hsps in wild-type and TU8 mutant cells. Leaf mesophyll cells were treated for 30 min at 38°C, for 30 min at 39°C, and for 2 h at 40°C. After fixation cells were incubated with antibodies against Hsp17.6 or Hsp90. The antibody-protein complex was detected with an FITC-conjugated secondary antibody and analyzed using confocal laserscan microscopy.
