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. Author manuscript; available in PMC: 2019 Apr 16.
Published in final edited form as: Mol Omics. 2018 Apr 16;14(2):109–120. doi: 10.1039/c8mo00011e

Figure 1.

Figure 1

Aqueous two-phase system mediated cell printing generates controlled size colonies. (a) Slot pins loaded with mESCs (beige) in the DEX phase is lowered on to PA6 cells (green) immersed in the PEG phase. (b, d) Pins content is autonomously dispensed to form isolated drops confining mESCs. (c) Printed mESCs attach to the stromal layer and proliferate to form a single colony. (e) A colony on day 8 of culture. (f) Neurite processes extend out from differentiating cells in mESC colonies. (g) Day 8 measured diameter of colonies formed with a density of 100, 250, and 500 mESCs. (h–j) Immunostained images of TuJ-positive colonies of 3 different sizes on day 8 of culture. (k) Day 8 measured total neurites density normalized with colony perimeter. * p < 0.01. n=18