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. Author manuscript; available in PMC: 2018 Apr 18.
Published in final edited form as: Stem Cell Res. 2018 Feb 28;28:29–32. doi: 10.1016/j.scr.2018.01.021

Table 1.

Characterization and validation.

Classification Test Result Data
Morphology Photography Normal Fig. 1 panel D
Phenotype Immunocytochemistry
RT-qPCR
NANOG, OCT4, SSEA4, TRA-1-81 and AP-positive.
Comparable of NANOG, OCT4, SOX2, DPPA4, and REX1 expression with H1.
Fig. 1 panel D
Fig. 1 panel E
Genotype Karyotype (G-banding) and resolution 46 XY
Resolution: 450–500
Fig. 1 panel H
Identity Microsatellite PCR (mPCR) OR
STR analysis
N/A
14/14 sites matched
N/A
Supplementary Table S1
Mutation analysis (IF APPLICABLE) Sequencing
Southern Blot OR WGS
Heterozygous deletion of 50 bp in RB1 exon 16.
N/A
Fig. 1 panel B
N/A
Microbiology and virology Mycoplasma Mycoplasma test shows negative. Fig. 1 panel G
Differentiation potential Directed in vitro differentiation Expression of SOX17 and HNF4A in HPCs, CD105 and CD166 in MSCs, and PAX6 and NESTIN in NPCs. Fig. 1 panel I, J, and K
Donor screening (OPTIONAL) HIV 1 + 2 Hepatitis B, Hepatitis C N/A N/A
Genotype additional info (OPTIONAL) Blood group genotyping
HLA tissue typing
N/A
N/A
N/A
N/A