Table 1.

Characterization and validation.

Classification	Test	Result	Data
Morphology	Photography	Normal	Fig. 1 panel D
Phenotype	Immunocytochemistry RT-qPCR	NANOG, OCT4, SSEA4, TRA-1-81 and AP-positive. Comparable of NANOG, OCT4, SOX2, DPPA4, and REX1 expression with H1.	Fig. 1 panel D Fig. 1 panel E
Genotype	Karyotype (G-banding) and resolution	46 XY Resolution: 450–500	Fig. 1 panel H
Identity	Microsatellite PCR (mPCR) OR STR analysis	N/A 14/14 sites matched	N/A Supplementary Table S1
Mutation analysis (IF APPLICABLE)	Sequencing Southern Blot OR WGS	Heterozygous deletion of 50 bp in RB1 exon 16. N/A	Fig. 1 panel B N/A
Microbiology and virology	Mycoplasma	Mycoplasma test shows negative.	Fig. 1 panel G
Differentiation potential	Directed in vitro differentiation	Expression of SOX17 and HNF4A in HPCs, CD105 and CD166 in MSCs, and PAX6 and NESTIN in NPCs.	Fig. 1 panel I, J, and K
Donor screening (OPTIONAL)	HIV 1 + 2 Hepatitis B, Hepatitis C	N/A	N/A
Genotype additional info (OPTIONAL)	Blood group genotyping HLA tissue typing	N/A N/A	N/A N/A