Generation of human embryonic stem cell line with heterozygous RB1 deletion by CRIPSR/Cas9 nickase

. Author manuscript; available in PMC: 2018 Apr 18.

Published in final edited form as: Stem Cell Res. 2018 Feb 28;28:29–32. doi: 10.1016/j.scr.2018.01.021

Unique stem cell line identifier	CDMLe001-A
Alternative name(s) of stem cell line	H1-RB1(E16)^−/+
Institution	The University of Texas Health Science Center at Houston
Contact information of distributor	dung-fang.lee@uth.tmc.edu
Type of cell line	Human embryonic stem cell line
Origin	hESC line H1 (WA01) from WiCell Institute
Additional origin info	Sex: Male (46, XY)
Cell Source	Human blastocyst
Clonality	Clonal
Method of reprogramming	N/A
Genetic Modification	Yes
Type of Modification	Deletion of 50 bp in RB1 exon 16.
Associated disease	Hereditary retinoblastoma
Gene/locus	13q14.1-14.2; RB1 exon 16
Method of modification	CRISPR/Cas9 nickase
Name of transgene or resistance	None
Inducible/constitutive system	None
Date archived/stock date	11/7/2017
Cell line repository/bank	None
Ethical approval	Cell lines were used according to institutional guidelines. UTHealth approval number: SCRO-16-01