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. 2000 Jul;123(3):971–978. doi: 10.1104/pp.123.3.971

Figure 3.

Figure 3

Accumulation of ACS and ACO transcripts in response to pollination in dl and Nr pistils. RNA was extracted from dl or Nr pistils at anthesis (A) and 4, 8, 12, 24, and 48 h after pollination. Pistils from mock-pollinated flowers (non-pollinated) were used as controls. Thirty micrograms of total RNA was hybridized to radiolabeled ACS gene-specific probes and used for RPA analysis. ACO gene expression was determined by RNA gel-blot analysis using 15 μg of total RNA. Gels were stained with ethidium bromide to ensure equal loading of the samples.