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Dynamic in-vivo imaging. a) Imaging in-vivo neurons expressing GCaMP6f. Imaging plane was fixed at ∼100μm away from the fiber tip. The fiber was inserted slowly into the tissue and videos were acquired at multiple depths. Images show the average intensity over time. b) Same location as (a), but showing standard deviation over time, which correlated directly with the amount of neural activity observed. c) Temporal traces from two example neurons after background subtraction.
