Transient transfection of hMSCs with a Sox9 promoter reporter. FGF2-expanded hMSC transiently transfected with a Sox9-luc reporter show an increased signal at day 5 compared with control-expanded cells, later manifested in an accelerated cell differentiation and ECM deposition at day 21, as previously reported.20,29
n = 5 (different donors), three representative samples shown. ECM, extracellular matrix; FGF2, fibroblast growth factor-2; hMSCs, human mesenchymal stem cells; Sox9, Sex Determining Region Y-Box 9. Color images available online at www.liebertpub.com/tea