FIG. 3.

Sox9 and AGG probes early predict the ultimate prochondrogenic effect of FGF2 exposure during hMSC expansion. (A) Secreted luciferase analyzed by BLI throughout the chondrogenic program shows an early Sox9 and AGG presence in FGF2-expanded hMSCs compared with control-expanded cells, evidenced by expected histological differences at the end of differentiation. (B) Quantification of the BLI signal, showing a similar pattern of expression with data obtained in a microarray analysis at similar time points and with multiple donors. n = 3; BLI run in duplicate for each donor; one representative donor shown; pellets correspond to one of the replicates of each donor; BLI quantification with all three donors incorporated; microarray data from two independent donors (used for reference only). *Statistical significance when p < 0.05. Color images available online at www.liebertpub.com/tea