FIG. 4.

AGG probe confirms the negative effect of FGF9 when present from day 0 in hMSC chondrogenesis. (A) Secreted luciferase analyzed by BLI throughout the chondrogenic program shows that AGG is not expressed at all in FGF2-expanded hMSC, subsequently exposed to FGF9 from day 0 of chondrogenesis, evidencing its negative effect on the differentiation program as reported in Correa et al.³⁰ (B) Quantification of the BLI signal and (C) Histology analysis of the resulting pellets (independent donors), confirming the negative effect of FGF9 when present throughout the differentiation program. n = 3; BLI run in triplicate for each donor; one representative donor shown; pellets correspond to one of the replicates of each donor; BLI quantification with all three donors incorporated; *statistical significance when p < 0.05. Color images available online at www.liebertpub.com/tea