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. 2018 Apr 1;24(7-8):607–615. doi: 10.1089/ten.tea.2017.0128

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© Benjamin Goyer et al. 2018; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are cited.

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FIG. 1. — Schematic representation of the tissue engineering protocol. (A) A healthy eye bank cornea was devitalized following three freeze-thaw cycles and used as a carrier for the engineering of a corneal endothelium using cultured cells isolated from FECD specimens. The same protocol was also used to engineer a corneal endothelium using healthy cells from normal eye bank corneas (not shown). (B) Histology cross-sections, trichrome Masson's staining. Top left: a native human eye bank cornea before devitalization; Top right: a devitalized human cornea before cell seeding. Note the absence of cells in the stroma and on Descemet's membrane; Bottom left: a corneal endothelium engineered using cells from patients with FECD; Bottom right: a corneal endothelium tissue engineered using healthy cells from an eye bank cornea. Scale bar: 20 μm. FECD, Fuchs endothelial corneal dystrophy.