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Extracellular matrix-related protein expression. Immunofluorescent staining (red) of type IV collagen (COL IV), laminin (LM) and fibronectin (FN) on healthy and FECD native tissues, as well as on corneal endothelia engineered using healthy and FECD cells. Cell nuclei were counterstained with Hoechst (blue). Descemet membranes are oriented stromal side up and endothelial side down. Scale bar: 20 μm.
