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. 2018 Apr 18;13(4):e0195744. doi: 10.1371/journal.pone.0195744

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© 2018 Bártolo et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — Evaluation of P3 anti-HIV1 activity in the presence of genital fluids: (A) SP, VFS and the combination of SP and VFS, (B) SP and VFS in different time points. (C) Effect of SP and VFS on P3 as determined by mass spectrometry. Solutions of P3 (366.4nM) were prepared in complete growth medium in the presence of 0.5% or 1% (v/v) SP, VFS, VFS with 0.5% or 1% (v/v) SP. The antiviral activity of each solution was evaluated with a single-round viral infectivity assay using TZM-bl reporter cells and HIV-1 primary isolate 93AOHDC250 in the presence of growth medium alone, SP and VFS to define the effects of biologic fluids on antiviral activity.