Figure 3.

LEACS4 expression in 60-DPA rin fruit treated with ethylene. Sixty-DPA rin fruit were held in air (−) or in 10 μL L ⁻¹ ethylene (+) for a further 4 d. Twenty-five micrograms of total RNA was hybridized with a radiolabeled RNA probe specific for LEACS4 and subjected to RPA analysis as described in the “Materials and Methods.” RNA gel-blot analysis of E4 expression is included as a positive control. Exposure times to x-ray film were as follows: LEACS4, 4 d; and E4, 16 h.