Table 2. Summary of sampling data and results obtained by lipl32 and 16S rRNA qPCR (presence/absence and concentration) for the three sites investigated.

			lipl32		16S rRNA
	Site	N of samples	Positive samples	Concentrationa	Positive samples	Concentrationa	Agreementb
Soil	A	34	9 (26%)	4 (4–17.8)	30 (88%)	2.13×102 (4–2.91×103)	0.09 ± 0.05 Poor
	B	14	3 (21%)	13 (4–4.31×102)	8 (57%)	96 (19–1.20×103)	0.34 ± 0.18 Fair
	C	22	10 (45%)	24 (4–4.09×102)	22 (100%)	6.95×102 (88.5–2.57×104)	0.00 ± 0.00 Poor
	All	70	22 (31%)	6 (4–4.31×102)	60 (86%)	4.16×102 (4–2.57×104)	0.14 ± 0.05 Poor
Sewage	C	8	7 (88%)	0.5 (0.5–0.9)	8 (100%)	2.09×102 (97.7–2.81×102)	0.00 ± 0.00 Poor

^aMedian concentration and range (GEq/g or GEq/mL)

^bCohen’s kappa coefficient between qualitative lipl32 and 16S rRNA qPCR results, standard error and strength of agreement.