Table 1.

Production of AAT protein from E. coli

Plasmid; Promoter	Expression Strain	Growth condition; Inducer	Induction Temp.; Time; Yield	Ref.
AAT expression and purification from soluble cell lysate
pOTSα (with N-term deleted AAT); λPL	AR120	32 °C; Thermal induction at A650 of 1.0	42 °C; 60–90 m; NR	[9]
λPL	TGE7213	15 1 culture at 30 °C; Thermal induction at A600 of 10	42 °C; 6 h; NR	[10]
pTermat; T7	BL21(DE3)	101 medium 37 °Ca; IPTG (0.1% w/v) at A600 of 10; Rifampicin at 0.1 mg/ml after 30 min of induction	37 °C; 3.5 h; 0.8 mg/g wet cell paste	[11]
pEAT8 (M2 AAT); T7	BL21(DE3)	Semi-defined medium; 0.4 mM IPTG at A600 of 1.4	37 °C; 3 h; NR	[12]
pMAL-C2x (MBP-AAT); pTac	BL21CPb	0.3 mM IPTG at A600 of 0.5b	37 °C; 8 h; 7–9 mg/l	[13]
a. pQE31 (His6-AAT); T5 b. pQE30 (His6-AAT); T5	SG13009 (pREP4)	2xYT medium; a. 1.0 mM IPTG at A600 of 0.8–1 b. 0.5 – 1.0 mM IPTG at A600 of 0.5 – 0.7	a. 30 °C; 3 h;NR b. 37 °C; 4 h;NR	[14] [15]
pEAT8–137 (M2 AAT); T7	BL21(DE3)	37 ° C, LB medium; 0.4 mM IPTG at A600 of 0.7–0.8	30 °C; 5 h; 6–7 mg/1	[16]c
AAT expression and purification from inclusion bodies
pαBcl (N-terminal deleted AAT); λPL	AR120	32 °C until A650 of 1.0; Thermal induction	42 °C; 60–90 m; NR	[9]
pEAT8 (M2 AAT); T7	BL21(DE3)	M9ZB medium; 0.4 mM IPTG at A600 of 0.8	40 °C; 3 h;NR	[17]
pTermat (Chimeric- AAT); T7	BL21(DE3)	37 °C; IPTG (0.1% w/v) at A600 of 10; Rifampicin at 0.1 mg/ml after 30 m of induction	37 °C; 3.5 h; 10–15 mg/1	[18]
pQE30(His6-Multi9AAT); T5	SG-13009	M9 Minimal medium; 1 mM IPTG at A600 of 0.5	4.5 h; NR	[19]
pTermat; T7	BL21(DE3)	2xYT medium; 0.5–1.0 mM IPTG at A600 of 0.5–0.7; Rifampicin at 0.05 mg/ml after 30 m of induction	37 °C; 4 h; NR	[15]

^aDefined medium for fermentation

^bOptimized strain and growth condition from [13]

^cCurrent Study

NR not reported