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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Virology. 2018 Jan 6;515:235–242. doi: 10.1016/j.virol.2017.12.026

Figure 3. Immunoprecipitation of ZIKV proteins by BLT mouse antisera.

Figure 3

Control uninfected and ZIKV infected Vero cells were radiolabeled with 35S-methionine/cysteine. Viral proteins were immunoprecipitated from the labeled cell lysates by using BLT mouse or human antisera and resolved on SDS-PAGE gels as described in Methods. Uninfected and infected ZIKV cell lysates immunoprecipitated by: lanes (a) and (b), uninfected and infected cell lysates with normal control human sera. Lanes (c) and (d), uninfected and infected cell lysates with ZIKV-positive human antisera. Lanes (e) and (f), uninfected and infected cell lysates with uninfected control mouse sera. Lanes (g, i, k) and (h, j, l), uninfected and infected cell lysates with three representative BLT mouse antiserum. ZIKV envelope protein (50 kDa) and capsid (12 kDa) polypeptides are indicated by *.